田崎　隆史　タサキ タカフミ

研究者基本情報

研究

研究の概要:
1) N-degron pathwayの生理機能とその分子機構の解析 2) UBR4とHPVE7の相互作用によるがん化機構の解析 3) 大腸炎関連大腸がんモデルにおけるユビキチンリガーゼUBR4の役割

専門分野

• 分子生物学
  Molecular Biology
• ウイルス学
  腫瘍生物学
• 実験動物学

学歴

• 北海道大学 大学院 獣医学研究科
• 北海道大学 獣医学部

学位

• 博士(獣医学)

所属学協会

• 2011年日本実験動物医学会 会員
• 2012年日本獣医学会 会員
• 2012年日本分子生物学会 会員
• 2012年北陸実験動物研究会 役員
• 2012年日本ウイルス学会 会員
• 2014年日本実験動物学会 会員
• 2017年07月American Association for Laboratory Animal Science 会員
• 2022年日本実験動物医学会 評議員

経歴

• 2012年02月金沢医科大学 准教授
• 2005年09月- 2012年01月ピッツバーグ大学薬学部 学内講師
• 2002年09月- 2005年08月ピッツバーグ大学薬学部 研究員
• 1999年06月- 2002年08月カフィフォルニア工科大学生物学部 ポストドクター
• 1998年04月- 1999年03月北海道大学免疫科学研究所 研究員

研究活動情報

論文

• UBR4/POE facilitates secretory trafficking to maintain circadian clock synchrony, (Hegazi S), Cheng AH, Krupp JJ, Tasaki T, Liu J, Szulc DA, Ling HH, Rios Garcia J, Seecharran S, Basiri T, Amiri M, Anwar Z, Ahmad S, Nayal K, Sonenberg N, Liu BH, Cheng HM, Levine JD, Cheng HM, Nature Communications, 13:1594, 2022年03月
• Threshold for Millimeter‑Wave (60 GHz)‑Induced Ocular Injury, Masami Kojima, Takafumi Tasaki, Yukihisa Suzuki, Toshio Kamijo, Aki Hada, Alfred Kik, Masateru Ikehata, Hiroshi Sasaki, Journal of Infrared Millimeter and Terahertz Waves, 43:260-271, 2022年04月
  DOI:https://doi.org/10.1007/s10762-022-00850-w
• Characterization of JC Polyomavirus Derived from COS-IMRb Cells, (Souichi Nukuzuma), Chiyoko Nukuzuma, Masanori Kameoka, Shigeki Sugiura, Kazuo Nakamichi, Takafumi Tasaki, Koushi Hidaka, Tsutomu Takegami, Japanese Journal of Infectious Diseases, 74:48-53, 2021年01月
  DOI:https://doi.org/10.7883/yoken.JJID.2020.325
• Dielectric property measurements of corneal tissues for computational dosimetry of the eye in terahertz band in vivo and in vitro., (Maya Mizuno), Hideaki Kitahara, Kensuke Sasaki, Masahiko Tani, Masami Kojima, Yukihisa Suzuki, Takafumi Tasaki, Yoshinori Tatematsu, Masafumi Fukunari, Kanako Wake, Biomedical Optics Express, 12:1295-1307, 2021年02月
• Clinical Course of High-Frequency Millimeter-Wave (162 GHz) Induced Ocular Injuries and Investigation of Damage Thresholds, Masami Kojima, Yukihisa Suzuki, Takafumi Tasaki, Yoshinori Tatematsu, Maya Mizuno, Masafumi Fukunari, Hiroshi Sasaki, Journal of Infrared, Millimeter, and Terahertz Waves, 41:834-845, 2020年06月
  DOI:org/10.1007/s10762-020-00714-1
• Creating a Stable Short-term Housing Environment for Rabbits in a Cargo Van, Takafumi Tasaki, Masami Kojima, Yukihisa Suzuki, Yoshinori Tatematsu, Hiroshi Sasaki, Journal of American Association for Laboratory Animal Science, 58:456-461, 2019年07月
• Ocular Response to Millimeter Wave Exposure Under Different Levels of Humidity, Masami Kojima, Cheng-Yu Tsai, Yukihisa Suzuki, Kensuke Sasaki, Takafumi Tasaki, Masao Taki, Soichi Watanabe, Hiroshi Sasaki, Journal of Infrared, Millimeter, and Terahertz Waves, 40:574-584, 2019年05月
  DOI:10.1007/s10762-019-00586-0
• The N-recognin UBR4 of the N-end rule pathway is required for neurogenesis and homeostasis of cell surface proteins, (Sung Tae.Kim), (Yoon Jee.Lee), Takafumi.Tasaki, Joonsung.Hwang, Min Jueng.Kang, Eugene C.Yi, Bo Yeon.Kim, Yong Tae.Kwon, PLOS ONE, 13:e0202260, 2018年08月
• The N-recognin UBR4 of the N-end rule pathway is targeted to and required for the biogenesis of the early endosome, (Sung Tae.Kim), (Yoon Jee.Lee), Takafumi.Tasaki, Su Ran.Mun, Joonsung.Hwang, Min Jueng.Kang, Srinivasrao.Ganipisetti, Eugene C.Yi, Bo Yeon.Kim, Yong Tae.Kwon, JOURNAL OF CELL SCIENCE, 131:jcs217646, 2018年09月
• Establishment of COS-JC cells persistently producing archetype JC polyomavirus, (Souichi.Nukuzuma), Chiyoko.Nukuzuma, Masanori.Kameoka, Shigeki.Sugiura, Kazuo.Nakamichi, Takafumi.Tasaki, Koushi.Hidaka, Tsutomu.Takegami, MICROBIOLOGY AND IMMUNOLOGY, 62:524-530, 2018年08月
• Ocular Effects of Exposure to 40, 75, and 95 GHz Millimeter Waves, Masami Kojima, Yukihisa Suzuki, Kensuke Sasaki, Masao Taki, Kanako Wake, Soichi Watanabe, Maya Mizuno, Takafumi Tasaki, Hiroshi Sasaki, Journal of Infrared, Millimeter, and Terahertz Waves, 39:912-925, 2018年09月
  DOI:doi.org/10.1007/s1062-018-0497-z
• CPT11 prevents virus replication in JCI cells persistently infected with JC polyomavirus., (Souichi.Nukuzuma), Chiyoko.Nukuzuma, Masanori.Kameoka, Shigeki.Sugiura, Kazuo.Nakamichi, Takafumi.Tasaki, Tsutomu.Takegami, Microbiology and immunology, 61:232, 2017年
  DOI:10.1111/1348-0421.12486
• Impaired Japanese encephalitis virus replication in p62/SQSTM1 deficient mouse embryonic fibroblasts, T.Tasaki, S. Nukuzuma, T. Takegami, Microbiology and Immunology, 60:708-711, 2016年10月
  DOI:10.1111/1348-0421.12440
• Japanese encephalitis virus replication and inhibitory effect of shRNA in mice, M.Murakami, T.Tasaki, S.Nukuzuma, H.Minato, T.Nojima, Y.Ishigaki, T.Takegami, Advances in Microbiology, 6:462-470, 2016年
• Sphingomyelin generated by sphingomyelin synthase 1 is involved in attachment and infection with Japanese encephalitis virus, M.Taniguchi, T.Tasaki, H.Ninomiya, Y.Ueda, K.Kuremoto, S.Mitsutake, Y.Igarashi, T.Okazaki, T.Takegami, Scientific Reports, 6:37829-37829, 2016年11月
  DOI:10.1038/srep37829
• Suppressive effect of topoisomerase inhibitors on JC polyomavirus propagation in human neuroblastoma cells, (S.Nukuzuma), K.Nakamichi, M.Kameoka, S.Sugiura, C.Nukuzuma, T.Tasaki, T.Takegami, Microbiology and Immunology, 60:253-260, 2016年04月
• Replication of IMR-32-adapted JC virus clones in human embryonic kidney cells, (S.Nukuzuma), S.Sugiura, K.Nakamichi, M.Kameoka, C.Nukuzuma, T.Tasaki, T.Takegami, Microbiology and Immunology, 59:238-242, 2015年
  DOI:10.1111/1348-0421.12243
• TNF-α stimulates efficient JC virus replication in neuroblastoma cells, (S.Nukuzuma), K.Nakamichi, M.Kameoka, S.Sugiura, C.Nukuzuma, T.Tasaki, T.Takegami, Journal of medical virology., 86:2026-2032, 2014年12月
• The N-end rule proteolytic system in autophagy., (ST.Kim), T.Tasaki, A.Zakrzewska, YD.Yoo, KS.Sung, S-H.Kim, H.Cha-Molstad, J.Hwang, KA.Kim, BY.Kim, YT.Kwon, Autophagy, 9:1100-1103, 2013年07月
• The N-end rule and retroviral infection: no effect on integrase., (G.Boso), T.Tasaki, YT.Kwon, NV.Somia, Virology journal [electronic resource], 10:233;1-14, 2013年07月
• UBR box N-recognin-4 (UBR4), an N-recognin of the N-end rule pathway, and its role in yolk sac vascular development and autophagy, T.Tasaki, ST. Kim, A. Zakrzewska, BE. Lee, MJ. Kang, YD. Yoo, HJ. Cha-Molstad, J. Hwang, NK. Soung, KS. Sung, SH. Kim, MD. Nguyen, M. Sun, EC. Yi, BY. Kim, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America, 110:3800-3805, 2013年03月
• The N-End rule pathway, T.Tasaki, SM. Sriram, KS. Park, YT. Kwon, Annual review of biochemistry, 81:261-289, 2012年04月
• Ubiquitin ligase UBR3 regulates cellular levels of the essential DNA repair protein APE1 and is required for genome stability, (C. Meisenberg), PS. Tait, II. Dianova, K. Wright, MJ. Edelmann, N. Ternette, T.Tasaki, BM. Kessler, JL. Parsons, YT. Kwon, GL. Dianov, Nucleic acids research, 40:701-711, 2012年01月
• The substrate recognition domains of the N-end rule pathway, T.Tasaki, Zakrzewska A, DD. Dudgeon, Y. Jiang, JS. Lazo, YT. Kwon, The Journal of biological chemistry., 284:1884-1895, 2009年01月
• Synthetic heterovalent inhibitors targeting recognition E3 components of the N-end rule pathway, (MJ. Lee), K. Pal, T.Tasaki, S. Roy, Y. Jiang, JY. An, R. Banerjee, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America, 105: 100-105, 2008年01月
• The mammalian N-end rule pathway: new insights into its components and physiological roles, T.Tasaki, YT. Kwon, Trends Biochem Sci, 32:520-528, 2007年10月
• Biochemical and genetic studies of UBR3, a ubiquitin ligase with a function in olfactory and other sensory systems, T.Tasaki, R. Sohr, Z. Xia, R. Hellweg, H. Hortnagl, A. Varshavsky, YT. Kwon, The Journal of biological chemistry., 282:18510-18520, 2007年06月
• Impaired neurogenesis and cardiovascular development in mice lacking the E3 ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway, (JY. An), JW. Seo, T.Tasaki, MJ. Lee, A. Varshavsky, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America, 103: 6212-6217, 2006年04月
• A family of mammalian E3 ubiquitin ligases that contain the UBR box motif and recognize N-degrons, T.Tasaki, LC. Mulder, A. Iwamatsu, MJ. Lee, IV. Davydov, A. Varshavsky, M. Muesing, YT. Kwon, Molecular and cellular biology, 25:7120-7136, 2005年08月
• RGS4 and RGS5 are in vivo substrates of the N-end rule pathway, (MJ. Lee), T.Tasaki, K. Moroi, JY. An, S. Kimura, IV. Davydov, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America, 102: 15030-15035, 2005年10月
• Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway, (YT. Kwon), Z. Xia, JY. An, T.Tasaki, IV. Davydov, JW. Seo, J. Sheng, Y. Xie, A. Varshavsky, Molecular and cellular biology, 23: 8255-8271, 2003年11月
• Pairs of dipepetides synergistically activate the binding of substrate by ubiquitin ligase through dissociation of its autoinhibitory domain, (F. Du), F. Navarro-Garcia, Z. Xia, T.Tasaki, A. Varshavsky, Proceedings of the National Academy of Sciences of the United States of America, 99: 14110-14115, 2002年10月
• Inhibition of pseudorabies virus replication by a dominant-negative mutant of early protein 0 expressed in a tetracycline-regulated system, T.Tasaki, S. Taharaguchi, T. Kobayashi, S. Yoshino, E. Ono, Veterinary Microbiology, 78:195-203, 2001年02月
• Translational regulation of platelet-derived growth factor B chain (c-sis) mRNA by short open reading frames in the 5'-untranslated region, (H. Nikami), T. Kobayashi, T.Tasaki, S. Yoshino, S. Taharaguchi, E. Ono, Platelets, 11:166-70, 2000年05月
• Resistance to pseudorabies virus infection in transgenic mice expressing the chimeric transgene that represses the immediate-early gene transcription, (E. Ono), T.Tasaki, T. Kobayashi, S. Taharaguchi, H. Nikami, I. Miyoshi, N. Kasai, J. Arikawa, H. Kida, Y. Shimizu, Virology., 262:72-78, 1999年09月
• Decrease in hepatic CYP2C11 mRNA and increase in heme oxygenase activity after intracerebroventricular injection of bacterial endotoxin, (Shimamoto Y), Tasaki T, Kitamura H, Hirose K, Kazusaka A, Fujita S, J. Vet. Med. Sci., 61:609-13, 1999年06月
• Cytochrome P450 isoforms responsible for the N-deethylation and cyclohexane-hydroxylation of NS-21, (Nakamura A), Hirota T, Morino A, Imaoka S, Funae Y, Yamamoto Y, Tasaki T, Masuda M, Kazusaka A, Fujita S, Xenobiotica, 29:243-252, 1999年03月
• Pseudorabies virus (PRV) early protein 0 activates PRV gene transcription in combination with the immediate-early protein IE180 and enhances the infectivity of PRV genomic DNA, (E. Ono), H. Nikami, T.Tasaki, H. Kida, Veterinary Microbiology, 63:99-107, 1998年10月
• Regio- and stereoselectivity in propranolol metabolism by dog liver microsomes and the expressed dog CYP2D15, Tasaki T, Iwata H, Kazusaka A, Fujita S, Journal of biochemistry., 123:747-751, 1998年04月
• Molecular basis of the Dark Agouti rat drug oxidation polymorphism: importance of CYP2D1 and CYP2D2, (Yamamoto Y), Tasaki T, Nakamura A, Iwata H, Kazusaka A, Gonzalez FJ, Fujita S, Pharmacogenetics, 8:73-82, 1998年02月
• Expression and characterization of dog CYP2D15 using baculovirus expression system, Tasaki T, Nakamura A, Itoh S, Ohashi K, Yamamoto Y, Masuda M, Iwata H, Kazusaka A, Kamataki T, Fujita S, Journal of biochemistry., 123:162-168, 1998年01月
• Strain differences in age-associated change in testosterone 6β-hydroxylation in Wistar and Dark Agouti rats, (Maeda Y), Morita K, Tasaki T, Kazusaka A, Imaoka S, Funae Y, Fujita S, Environ Toxicol Pharmacol, 3:1-6, 1997年02月
• Impaired expression in DA rats of catalytically active P450BTL, immunochemically distinguishable from P4502D1: Immunological evidence with specific anti-peptide antibodies, (Yamamoto Yukio), Nakamura Akio, Tasaki Takafumi, Umeda Shin, Sugimoto Chihiro, Imaoka Susumu, Fujita Shoichi, Biomedical research (Tokyo, Japan), 17: 331-337, 1996年
• Purification and characterization of a dog cytochrome P450 isozyme belonging to the CYP2D subfamily and development of its antipeptide antibody, Nakamura A, Yamamoto Y, Tasaki T, Sugimoto C, Masuda M, Kazusaka A, Fujita S, Drug Metab Dispos, 23:1268-73, 1995年11月
• Anti-peptide antibodies to the P4502D subfamily in rat, dog and man, (Nakamura A), Yamamoto Y, Tasaki T, Sugimoto C, Masuda M, Kazusaka A, Fujita S, Xenobiotica, 25:1103-1109:1103-1109, 1995年11月
• Effects of High Temperature and High Humidity on the Degree of Ocular Damage Caused by 60 GHz Millimeter Wave Exposure, Kojima M, Tasaki T, Kamijo T, Hada A, Suzuki Y, Ikehata M, Sasaki H., Health Phys, 127:557-564, 2024年11月
  DOI:https://doi.org/10.1097/HP.0000000000001843

書籍等出版物

• Japanese Encephalitis Virus Infection and Replication: Biological Roles of Nonstructural Protein NS4a and The 3'-Untranslated Region in Persistent Infection
  Japanese Encephalitis
  T.Takegami, T.Tasaki, M.Murakami, Y.Ishigaki, M.Taniguchi, T.Nojima, S.Nukuzuma
  SMGroup (USA) 2015年08月

受賞

• 2012年09月 北國がん研究振興財団 北國がん基金助成金
  田崎　隆史,

競争的資金

• 大腸炎関連発癌に関与するユビキチンリガーゼUBR4の生理学的機能とその分子機構
  文部科学省：科学研究費　基盤研究(C)
  研究期間：2021年 - 2023年  
  代表者:田崎　隆史
• UBR4/p600-HPV16 E7相互作用による発がんメカニズム
  文部科学省：科学研究費　基盤研究（Ｃ）
  研究期間：2013年 - 2015年  
  代表者:田崎隆史
• ユビキチンリガーゼUBR4の組織特異的生理機能と分子機構
  文部科学省：科学研究費　基盤研究(C)
  研究期間：2018年 - 2020年  
  代表者:田崎　隆史