TASAKI Takafumi

Researcher Profile & Settings

Research

Overview of research:
1) Physiological function of the N-degron pathway 2) Role of UBR4-E7 interaction in the HPV-mediated carcinogenesis 3) Role of the ubiquitin ligase UBR4 in a mouse model of colitis-associated cancer

Special Field

• Molecular biology
• Virology

Education

• Hokkaido University Graduate School
• Hokkaido University

Association Memberships

• 2011- Japanese Association for Laboratory Animal Medicine (JALAM)
• 2012- The Japanese Society of Veterinary Science
• 2012- The Molecular Biology Society of Japan
• 2012- Hokuriku Laboratory Animal Research Association
• 2012- The Japanese Society for Virology
• 2014- Japanese Association for Laboratory Animal Science
• Jul. 2017- American Association for Laboratory Animal Science
• 2022- Japanese Association for Laboratory Animal Medicine (JALAM)

Academic & Professional Experience

• Feb. 2012Kanazawa Medical University Associate Professor
• Sep. 2005- Jan. 2012University of Pittsburgh, School of Pharmacy Senior Assistant Professor
• Sep. 2002- Aug. 2005University of Pittsburgh, School of Pharmacy Research Fellow
• Jun. 1999- Aug. 2002California Institute of Technology, Division of Biology Project Postdoctoral Fellow
• Apr. 1998- Mar. 1999Institute of Immunological Science, Hokkaido University Research Fellow

Research Activities

Published Papers

• UBR4/POE facilitates secretory trafficking to maintain circadian clock synchrony, (Hegazi S), Cheng AH, Krupp JJ, Tasaki T, Liu J, Szulc DA, Ling HH, Rios Garcia J, Seecharran S, Basiri T, Amiri M, Anwar Z, Ahmad S, Nayal K, Sonenberg N, Liu BH, Cheng HM, Levine JD, Cheng HM, Nature Communications, 13:1594, Mar. 2022
• Threshold for Millimeter‑Wave (60 GHz)‑Induced Ocular Injury, Masami Kojima, Takafumi Tasaki, Yukihisa Suzuki, Toshio Kamijo, Aki Hada, Alfred Kik, Masateru Ikehata, Hiroshi Sasaki, Journal of Infrared Millimeter and Terahertz Waves, 43:260-271, Apr. 2022
  DOI:https://doi.org/10.1007/s10762-022-00850-w
• Characterization of JC Polyomavirus Derived from COS-IMRb Cells, (Souichi Nukuzuma), Chiyoko Nukuzuma, Masanori Kameoka, Shigeki Sugiura, Kazuo Nakamichi, Takafumi Tasaki, Koushi Hidaka, Tsutomu Takegami, Japanese Journal of Infectious Diseases, 74:48-53, Jan. 2021
  DOI:https://doi.org/10.7883/yoken.JJID.2020.325
• Dielectric property measurements of corneal tissues for computational dosimetry of the eye in terahertz band in vivo and in vitro., (Maya Mizuno), Hideaki Kitahara, Kensuke Sasaki, Masahiko Tani, Masami Kojima, Yukihisa Suzuki, Takafumi Tasaki, Yoshinori Tatematsu, Masafumi Fukunari, Kanako Wake, Biomedical Optics Express, 12:1295-1307, Feb. 2021
• Clinical Course of High-Frequency Millimeter-Wave (162 GHz) Induced Ocular Injuries and Investigation of Damage Thresholds, Masami Kojima, Yukihisa Suzuki, Takafumi Tasaki, Yoshinori Tatematsu, Maya Mizuno, Masafumi Fukunari, Hiroshi Sasaki, Journal of Infrared, Millimeter, and Terahertz Waves, 41:834-845, Jun. 2020
  DOI:org/10.1007/s10762-020-00714-1
• Creating a Stable Short-term Housing Environment for Rabbits in a Cargo Van, Takafumi Tasaki, Masami Kojima, Yukihisa Suzuki, Yoshinori Tatematsu, Hiroshi Sasaki, Journal of American Association for Laboratory Animal Science, 58:456-461, Jul. 2019
• Ocular Response to Millimeter Wave Exposure Under Different Levels of Humidity, Masami Kojima, Cheng-Yu Tsai, Yukihisa Suzuki, Kensuke Sasaki, Takafumi Tasaki, Masao Taki, Soichi Watanabe, Hiroshi Sasaki, Journal of Infrared, Millimeter, and Terahertz Waves, 40:574-584, May 2019
  DOI:10.1007/s10762-019-00586-0
• The N-recognin UBR4 of the N-end rule pathway is required for neurogenesis and homeostasis of cell surface proteins, (Sung Tae.Kim), (Yoon Jee.Lee), Takafumi.Tasaki, Joonsung.Hwang, Min Jueng.Kang, Eugene C.Yi, Bo Yeon.Kim, Yong Tae.Kwon, PLOS ONE, 13:e0202260, Aug. 2018
• The N-recognin UBR4 of the N-end rule pathway is targeted to and required for the biogenesis of the early endosome, (Sung Tae.Kim), (Yoon Jee.Lee), Takafumi.Tasaki, Su Ran.Mun, Joonsung.Hwang, Min Jueng.Kang, Srinivasrao.Ganipisetti, Eugene C.Yi, Bo Yeon.Kim, Yong Tae.Kwon, JOURNAL OF CELL SCIENCE, 131:jcs217646, Sep. 2018
• Establishment of COS-JC cells persistently producing archetype JC polyomavirus, (Souichi.Nukuzuma), Chiyoko.Nukuzuma, Masanori.Kameoka, Shigeki.Sugiura, Kazuo.Nakamichi, Takafumi.Tasaki, Koushi.Hidaka, Tsutomu.Takegami, MICROBIOLOGY AND IMMUNOLOGY, 62:524-530, Aug. 2018
• Ocular Effects of Exposure to 40, 75, and 95 GHz Millimeter Waves, Masami Kojima, Yukihisa Suzuki, Kensuke Sasaki, Masao Taki, Kanako Wake, Soichi Watanabe, Maya Mizuno, Takafumi Tasaki, Hiroshi Sasaki, Journal of Infrared, Millimeter, and Terahertz Waves, 39:912-925, Sep. 2018
  DOI:doi.org/10.1007/s1062-018-0497-z
• CPT11 prevents virus replication in JCI cells persistently infected with JC polyomavirus., (Souichi.Nukuzuma), Chiyoko.Nukuzuma, Masanori.Kameoka, Shigeki.Sugiura, Kazuo.Nakamichi, Takafumi.Tasaki, Tsutomu.Takegami, Microbiology and immunology, 61:232, 2017
  DOI:10.1111/1348-0421.12486
• Impaired Japanese encephalitis virus replication in p62/SQSTM1 deficient mouse embryonic fibroblasts, T.Tasaki, S. Nukuzuma, T. Takegami, Microbiology and Immunology, 60:708-711, Oct. 2016
  DOI:10.1111/1348-0421.12440
• Japanese encephalitis virus replication and inhibitory effect of shRNA in mice, M.Murakami, T.Tasaki, S.Nukuzuma, H.Minato, T.Nojima, Y.Ishigaki, T.Takegami, Advances in Microbiology, 6:462-470, 2016
• Sphingomyelin generated by sphingomyelin synthase 1 is involved in attachment and infection with Japanese encephalitis virus, M.Taniguchi, T.Tasaki, H.Ninomiya, Y.Ueda, K.Kuremoto, S.Mitsutake, Y.Igarashi, T.Okazaki, T.Takegami, Scientific Reports, 6:37829-37829, Nov. 2016
  DOI:10.1038/srep37829
• Suppressive effect of topoisomerase inhibitors on JC polyomavirus propagation in human neuroblastoma cells, (S.Nukuzuma), K.Nakamichi, M.Kameoka, S.Sugiura, C.Nukuzuma, T.Tasaki, T.Takegami, Microbiology and Immunology, 60:253-260, Apr. 2016
• Replication of IMR-32-adapted JC virus clones in human embryonic kidney cells, (S.Nukuzuma), S.Sugiura, K.Nakamichi, M.Kameoka, C.Nukuzuma, T.Tasaki, T.Takegami, Microbiology and Immunology, 59:238-242, 2015
  DOI:10.1111/1348-0421.12243
• TNF-α stimulates efficient JC virus replication in neuroblastoma cells, (S.Nukuzuma), K.Nakamichi, M.Kamaoka, S.Sugiura, C.Nukuzuma, T.Tasaki, T.Takegami, Journal of medical virology., 86:2026-2032, Dec. 2014
• The N-end rule proteolytic system in autophagy., (ST.Kim), T.Tasaki, A.Zakrzewska, YD.Yoo, KS.Sung, S-H.Kim, H.Cha-Molstad, J.Hwang, KA.Kim, BY.Kim, YT.Kwon, Autophagy, 9:1100-1103, Jul. 2013
• The N-end rule and retroviral infection: no effect on integrase., (G.Boso), T.Tasaki, YT.Kwon, NV.Somia, Virology journal [electronic resource], 10:233;1-14, Jul. 2013
• UBR box N-recognin-4 (UBR4), an N-recognin of the N-end rule pathway, and its role in yolk sac vascular development and autophagy, T.Tasaki, ST. Kim, A. Zakrzewska, BE. Lee, MJ. Kang, YD. Yoo, HJ. Cha-Molstad, J. Hwang, NK. Soung, KS. Sung, SH. Kim, MD. Nguyen, M. Sun, EC. Yi, BY. Kim, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America, 110:3800-3805, Mar. 2013
• The N-End rule pathway, T.Tasaki, SM. Sriram, KS. Park, YT. Kwon, Annual review of biochemistry, 81:261-289, Apr. 2012
• Ubiquitin ligase UBR3 regulates cellular levels of the essential DNA repair protein APE1 and is required for genome stability, (C. Meisenberg), PS. Tait, II. Dianova, K. Wright, MJ. Edelmann, N. Ternette, T.Tasaki, BM. Kessler, JL. Parsons, YT. Kwon, GL. Dianov, Nucleic acids research, 40:701-711, Jan. 2012
• The substrate recognition domains of the N-end rule pathway, T.Tasaki, Zakrzewska A, DD. Dudgeon, Y. Jiang, JS. Lazo, YT. Kwon, The Journal of biological chemistry., 284:1884-1895, Jan. 2009
• Synthetic heterovalent inhibitors targeting recognition E3 components of the N-end rule pathway, (MJ. Lee), K. Pal, T.Tasaki, S. Roy, Y. Jiang, JY. An, R. Banerjee, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America, 105: 100-105, Jan. 2008
• The mammalian N-end rule pathway: new insights into its components and physiological roles, T.Tasaki, YT. Kwon, Trends Biochem Sci, 32:520-528, Oct. 2007
• Biochemical and genetic studies of UBR3, a ubiquitin ligase with a function in olfactory and other sensory systems, T.Tasaki, R. Sohr, Z. Xia, R. Hellweg, H. Hortnagl, A. Varshavsky, YT. Kwon, The Journal of biological chemistry., 282:18510-18520, Jun. 2007
• Impaired neurogenesis and cardiovascular development in mice lacking the E3 ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway, (JY. An), JW. Seo, T.Tasaki, MJ. Lee, A. Varshavsky, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America, 103: 6212-6217, Apr. 2006
• A family of mammalian E3 ubiquitin ligases that contain the UBR box motif and recognize N-degrons, T.Tasaki, LC. Mulder, A. Iwamatsu, MJ. Lee, IV. Davydov, A. Varshavsky, M. Muesing, YT. Kwon, Molecular and cellular biology, 25:7120-7136, Aug. 2005
• RGS4 and RGS5 are in vivo substrates of the N-end rule pathway, (MJ. Lee), T.Tasaki, K. Moroi, JY. An, S. Kimura, IV. Davydov, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America, 102: 15030-15035, Oct. 2005
• Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway, (YT. Kwon), Z. Xia, JY. An, T.Tasaki, IV. Davydov, JW. Seo, J. Sheng, Y. Xie, A. Varshavsky, Molecular and cellular biology, 23: 8255-8271, Nov. 2003
• Pairs of dipepetides synergistically activate the binding of substrate by ubiquitin ligase through dissociation of its autoinhibitory domain, (F. Du), F. Navarro-Garcia, Z. Xia, T.Tasaki, A. Varshavsky, Proceedings of the National Academy of Sciences of the United States of America, 99: 14110-14115, Oct. 2002
• Inhibition of pseudorabies virus replication by a dominant-negative mutant of early protein 0 expressed in a tetracycline-regulated system, T.Tasaki, S. Taharaguchi, T. Kobayashi, S. Yoshino, E. Ono, Veterinary Microbiology, 78:195-203, Feb. 2001
• Translational regulation of platelet-derived growth factor B chain (c-sis) mRNA by short open reading frames in the 5'-untranslated region, (H. Nikami), T. Kobayashi, T.Tasaki, S. Yoshino, S. Taharaguchi, E. Ono, Platelets, 11:166-70, May 2000
• Resistance to pseudorabies virus infection in transgenic mice expressing the chimeric transgene that represses the immediate-early gene transcription, (E. Ono), T.Tasaki, T. Kobayashi, S. Taharaguchi, H. Nikami, I. Miyoshi, N. Kasai, J. Arikawa, H. Kida, Y. Shimizu, Virology., 262:72-78, Sep. 1999
• Decrease in hepatic CYP2C11 mRNA and increase in heme oxygenase activity after intracerebroventricular injection of bacterial endotoxin, (Shimamoto Y), Tasaki T, Kitamura H, Hirose K, Kazusaka A, Fujita S, J. Vet. Med. Sci., 61:609-13, Jun. 1999
• Cytochrome P450 isoforms responsible for the N-deethylation and cyclohexane-hydroxylation of NS-21, (Nakamura A), Hirota T, Morino A, Imaoka S, Funae Y, Yamamoto Y, Tasaki T, Masuda M, Kazusaka A, Fujita S, Xenobiotica, 29:243-252, Mar. 1999
• Pseudorabies virus (PRV) early protein 0 activates PRV gene transcription in combination with the immediate-early protein IE180 and enhances the infectivity of PRV genomic DNA, (E. Ono), H. Nikami, T.Tasaki, H. Kida, Veterinary Microbiology, 63:99-107, Oct. 1998
• Regio- and stereoselectivity in propranolol metabolism by dog liver microsomes and the expressed dog CYP2D15, Tasaki T, Iwata H, Kazusaka A, Fujita S, Journal of biochemistry., 123:747-751, Apr. 1998
• Molecular basis of the Dark Agouti rat drug oxidation polymorphism: importance of CYP2D1 and CYP2D2, (Yamamoto Y), Tasaki T, Nakamura A, Iwata H, Kazusaka A, Gonzalez FJ, Fujita S, Pharmacogenetics, 8:73-82, Feb. 1998
• Expression and characterization of dog CYP2D15 using baculovirus expression system, Tasaki T, Nakamura A, Itoh S, Ohashi K, Yamamoto Y, Masuda M, Iwata H, Kazusaka A, Kamataki T, Fujita S, Journal of biochemistry., 123:162-168, Jan. 1998
• Strain differences in age-associated change in testosterone 6β-hydroxylation in Wistar and Dark Agouti rats, (Maeda Y), Morita K, Tasaki T, Kazusaka A, Imaoka S, Funae Y, Fujita S, Environ Toxicol Pharmacol, 3:1-6, Feb. 1997
• Impaired expression in DA rats of catalytically active P450BTL, immunochemically distinguishable from P4502D1: Immunological evidence with specific anti-peptide antibodies, (Yamamoto, Y), Nakamura, A, T Tasaki, Umeda Shin, Sugimoto, C, Imaoka, S, Fujita, S, Biomedical research (Tokyo, Japan), 17: 331-337, 1996
• Purification and characterization of a dog cytochrome P450 isozyme belonging to the CYP2D subfamily and development of its antipeptide antibody, Nakamura A, Yamamoto Y, Tasaki T, Sugimoto C, Masuda M, Kazusaka A, Fujita S, Drug Metab Dispos, 23:1268-73, Nov. 1995
• Anti-peptide antibodies to the P4502D subfamily in rat, dog and man, (Nakamura A), Yamamoto Y, Tasaki T, Sugimoto C, Masuda M, Kazusaka A, Fujita S, Xenobiotica, 25:1103-1109:1103-1109, Nov. 1995
• Effects of High Temperature and High Humidity on the Degree of Ocular Damage Caused by 60 GHz Millimeter Wave Exposure, Kojima M, Tasaki T, Kamijo T, Hada A, Suzuki Y, Ikehata M, Sasaki H., Health Phys, 127:557-564, Nov. 2024
  DOI:https://doi.org/10.1097/HP.0000000000001843

Books etc

• Japanese Encephalitis Virus Infection and Replication: Biological Roles of Nonstructural Protein NS4a and The 3'-Untranslated Region in Persistent Infection
  Japanese Encephalitis
  T.Takegami, T.Tasaki, M.Murakami, Y.Ishigaki, M.Taniguchi, T.Nojima, S.Nukuzuma
  SMGroup (USA)  Aug. 2015
  www.smgebooks.com