TASAKI Takafumi

FacultyDepartment of Life Sciences, Department of Medical Zoology
PositionAssociate Professor

Researcher Profile & Settings


    Overview of research:
    1) Physiological function of the N-end rule pathway 2) Role of UBR4-E7 interaction in the HPV-mediated carcinogenesis 3) Role of lysosomal degradation system in the replication of Japanese encephalitis virus

Special Field

  • Molecular biology
  • Virology


  • Hokkaido University Graduate School
  • Hokkaido University

Association Memberships

  • 2011Japanese Association for Laboratory Animal Medicine (JALAM)
  • 2014Japanese Association for Laboratory Animal Science
  • 2012The Japanese Society of Veterinary Science
  • 2012The Molecular Biology Society of Japan
  • 2012Hokuriku Laboratory Animal Research Association
  • 2012The Japanese Society for Virology
  • Jul. 2017American Association for Laboratory Animal Science

Academic & Professional Experience

  • Feb. 2012Kanazawa Medical University Associate Professor
  • Sep. 2005Jan. 2012University of Pittsburgh, School of Pharmacy Research Assistant Professor
  • Sep. 2002Aug. 2005University of Pittsburgh, School of Pharmacy Research Associate
  • Jun. 1999Aug. 2002California Institute of Technology, Division of Biology Postdoctoral Scholar in Biology
  • Apr. 1998Mar. 1999Institute of Immunological Science, Hokkaido University Research Associate

Research Activities

Published Papers

  • The N-recognin UBR4 of the N-end rule pathway is required for neurogenesis and homeostasis of cell surface proteins, (Sung Tae.Kim), (Yoon Jee.Lee), Takafumi.Tasaki, Joonsung.Hwang, Min Jueng.Kang, Eugene C.Yi, Bo Yeon.Kim, Yong Tae.Kwon, PLOS ONE13:e0202260Aug. 2018
  • The N-recognin UBR4 of the N-end rule pathway is targeted to and required for the biogenesis of the early endosome, (Sung Tae.Kim), (Yoon Jee.Lee), Takafumi.Tasaki, Su Ran.Mun, Joonsung.Hwang, Min Jueng.Kang, Srinivasrao.Ganipisetti, Eugene C.Yi, Bo Yeon.Kim, Yong Tae.Kwon, JOURNAL OF CELL SCIENCE131:jcs217646Sep. 2018
  • Establishment of COS-JC cells persistently producing archetype JC polyomavirus, (Souichi.Nukuzuma), Chiyoko.Nukuzuma, Masanori.Kameoka, Shigeki.Sugiura, Kazuo.Nakamichi, Takafumi.Tasaki, Koushi.Hidaka, Tsutomu.Takegami, MICROBIOLOGY AND IMMUNOLOGY62:524-530Aug. 2018
  • Ocular Effects of Exposure to 40, 75, and 95 GHz Millimeter Waves, Masami Kojima, Yukihisa Suzuki, Kensuke Sasaki, Masao Taki, Kanako Wake, Soichi Watanabe, Maya Mizuno, Takafumi Tasaki, Hiroshi Sasaki, Journal of Infrared, Millimeter, and Terahertz Waves39:912-925Sep. 2018
  • CPT11 prevents virus replication in JCI cells persistently infected with JC polyomavirus., (Souichi.Nukuzuma), Chiyoko.Nukuzuma, Masanori.Kameoka, Shigeki.Sugiura, Kazuo.Nakamichi, Takafumi.Tasaki, Tsutomu.Takegami, Microbiology and immunology61:2322017
  • Japanese encephalitis virus replication and inhibitory effect of shRNA in mice, M.Murakami, T.Tasaki, S.Nukuzuma, H.Minato, T.Nojima, Y.Ishigaki, T.Takegami, Advances in Microbiology6:462-4702016
  • Sphingomyelin generated by sphingomyelin synthase 1 is involved in attachment and infection with Japanese encephalitis virus, M.Taniguchi, T.Tasaki, H.Ninomiya, Y.Ueda, K.Kuremoto, S.Mitsutake, Y.Igarashi, T.Okazaki, T.Takegami, Scientific Reports6:37829-37829Nov. 2016
  • Suppressive effect of topoisomerase inhibitors on JC polyomavirus propagation in human neuroblastoma cells, (S.Nukuzuma), K.Nakamichi, M.Kameoka, S.Sugiura, C.Nukuzuma, T.Tasaki, T.Takegami, Microbiology and Immunology60:253-260Apr. 2016
  • Impaired Japanese encephalitis virus replication in p62/SQSTM1 deficient mouse embryonic fibroblasts, T.Tasaki, S. Nukuzuma, T. Takegami, Microbiology and Immunology60:708-711Oct. 2016
  • Replication of IMR-32-adapted JC virus clones in human embryonic kidney cells, (S.Nukuzuma), S.Sugiura, K.Nakamichi, M.Kameoka, C.Nukuzuma, T.Tasaki, T.Takegami, Microbiology and Immunology59:238-2422015
  • TNF-α stimulates efficient JC virus replication in neuroblastoma cells, (S.Nukuzuma), K.Nakamichi, M.Kamaoka, S.Sugiura, C.Nukuzuma, T.Tasaki, T.Takegami, Journal of medical virology.86:2026-2032Dec. 2014
  • The N-end rule proteolytic system in autophagy., (ST.Kim), T.Tasaki, A.Zakrzewska, YD.Yoo, KS.Sung, S-H.Kim, H.Cha-Molstad, J.Hwang, KA.Kim, BY.Kim, YT.Kwon, Autophagy9:1100-1103Jul. 2013
  • The N-end rule and retroviral infection: no effect on integrase., (G.Boso), T.Tasaki, YT.Kwon, NV.Somia, Virology journal [electronic resource]10:233;1-14Jul. 2013
  • UBR box N-recognin-4 (UBR4), an N-recognin of the N-end rule pathway, and its role in yolk sac vascular development and autophagy, T.Tasaki, ST. Kim, A. Zakrzewska, BE. Lee, MJ. Kang, YD. Yoo, HJ. Cha-Molstad, J. Hwang, NK. Soung, KS. Sung, SH. Kim, MD. Nguyen, M. Sun, EC. Yi, BY. Kim, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America110:3800-3805Mar. 2013
  • The N-End rule pathway, T.Tasaki, SM. Sriram, KS. Park, YT. Kwon, Annual review of biochemistry81:261-289Apr. 2012
  • Ubiquitin ligase UBR3 regulates cellular levels of the essential DNA repair protein APE1 and is required for genome stability, (C. Meisenberg), PS. Tait, II. Dianova, K. Wright, MJ. Edelmann, N. Ternette, T.Tasaki, BM. Kessler, JL. Parsons, YT. Kwon, GL. Dianov, Nucleic acids research40:701-711Jan. 2012
  • The substrate recognition domains of the N-end rule pathway, T.Tasaki, Zakrzewska A, DD. Dudgeon, Y. Jiang, JS. Lazo, YT. Kwon, The Journal of biological chemistry.284:1884-1895Jan. 2009
  • Synthetic heterovalent inhibitors targeting recognition E3 components of the N-end rule pathway, (MJ. Lee), K. Pal, T.Tasaki, S. Roy, Y. Jiang, JY. An, R. Banerjee, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America105: 100-105Jan. 2008
  • The mammalian N-end rule pathway: new insights into its components and physiological roles, T.Tasaki, YT. Kwon, Trends Biochem Sci32:520-528Oct. 2007
  • Biochemical and genetic studies of UBR3, a ubiquitin ligase with a function in olfactory and other sensory systems, T.Tasaki, R. Sohr, Z. Xia, R. Hellweg, H. Hortnagl, A. Varshavsky, YT. Kwon, The Journal of biological chemistry.282:18510-18520Jun. 2007
  • Impaired neurogenesis and cardiovascular development in mice lacking the E3 ubiquitin ligases UBR1 and UBR2 of the N-end rule pathway, (JY. An), JW. Seo, T.Tasaki, MJ. Lee, A. Varshavsky, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America103: 6212-6217Apr. 2006
  • RGS4 and RGS5 are in vivo substrates of the N-end rule pathway, (MJ. Lee), T.Tasaki, K. Moroi, JY. An, S. Kimura, IV. Davydov, YT. Kwon, Proceedings of the National Academy of Sciences of the United States of America102: 15030-15035Oct. 2005
  • A family of mammalian E3 ubiquitin ligases that contain the UBR box motif and recognize N-degrons, T.Tasaki, LC. Mulder, A. Iwamatsu, MJ. Lee, IV. Davydov, A. Varshavsky, M. Muesing, YT. Kwon, Molecular and cellular biology25:7120-7136Aug. 2005
  • Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway, (YT. Kwon), Z. Xia, JY. An, T.Tasaki, IV. Davydov, JW. Seo, J. Sheng, Y. Xie, A. Varshavsky, Molecular and cellular biology23: 8255-8271Nov. 2003
  • Pairs of dipepetides synergistically activate the binding of substrate by ubiquitin ligase through dissociation of its autoinhibitory domain, (F. Du), F. Navarro-Garcia, Z. Xia, T.Tasaki, A. Varshavsky, Proceedings of the National Academy of Sciences of the United States of America99: 14110-14115Oct. 2002
  • Inhibition of pseudorabies virus replication by a dominant-negative mutant of early protein 0 expressed in a tetracycline-regulated system, T.Tasaki, S. Taharaguchi, T. Kobayashi, S. Yoshino, E. Ono, Veterinary Microbiology78:195-203Feb. 2001
  • Translational regulation of platelet-derived growth factor B chain (c-sis) mRNA by short open reading frames in the 5'-untranslated region, (H. Nikami), T. Kobayashi, T.Tasaki, S. Yoshino, S. Taharaguchi, E. Ono, Platelets11:166-70May 2000
  • Resistance to pseudorabies virus infection in transgenic mice expressing the chimeric transgene that represses the immediate-early gene transcription, (E. Ono), T.Tasaki, T. Kobayashi, S. Taharaguchi, H. Nikami, I. Miyoshi, N. Kasai, J. Arikawa, H. Kida, Y. Shimizu, Virology.262:72-78Sep. 1999
  • Decrease in hepatic CYP2C11 mRNA and increase in heme oxygenase activity after intracerebroventricular injection of bacterial endotoxin, (Shimamoto Y), Tasaki T, Kitamura H, Hirose K, Kazusaka A, Fujita S, J. Vet. Med. Sci.61:609-13Jun. 1999
  • Cytochrome P450 isoforms responsible for the N-deethylation and cyclohexane-hydroxylation of NS-21, (Nakamura A), Hirota T, Morino A, Imaoka S, Funae Y, Yamamoto Y, Tasaki T, Masuda M, Kazusaka A, Fujita S, Xenobiotica29:243-252Mar. 1999
  • Pseudorabies virus (PRV) early protein 0 activates PRV gene transcription in combination with the immediate-early protein IE180 and enhances the infectivity of PRV genomic DNA, (E. Ono), H. Nikami, T.Tasaki, H. Kida, Veterinary Microbiology63:99-107Oct. 1998
  • Regio- and stereoselectivity in propranolol metabolism by dog liver microsomes and the expressed dog CYP2D15, Tasaki T, Iwata H, Kazusaka A, Fujita S, Journal of biochemistry.123:747-751Apr. 1998
  • Molecular basis of the Dark Agouti rat drug oxidation polymorphism: importance of CYP2D1 and CYP2D2, (Yamamoto Y), Tasaki T, Nakamura A, Iwata H, Kazusaka A, Gonzalez FJ, Fujita S, Pharmacogenetics8:73-82Feb. 1998
  • Expression and characterization of dog CYP2D15 using baculovirus expression system, Tasaki T, Nakamura A, Itoh S, Ohashi K, Yamamoto Y, Masuda M, Iwata H, Kazusaka A, Kamataki T, Fujita S, Journal of biochemistry.123:162-168Jan. 1998
  • Strain differences in age-associated change in testosterone 6β-hydroxylation in Wistar and Dark Agouti rats, (Maeda Y), Morita K, Tasaki T, Kazusaka A, Imaoka S, Funae Y, Fujita S, Environ Toxicol Pharmacol3:1-6Feb. 1997
  • Impaired expression in DA rats of catalytically active P450BTL, immunochemically distinguishable from P4502D1: Immunological evidence with specific anti-peptide antibodies, (Yamamoto, Y), Nakamura, A, T Tasaki, Umeda Shin, Sugimoto, C, Imaoka, S, Fujita, S, Biomedical research (Tokyo, Japan)17: 331-3371996
  • Purification and characterization of a dog cytochrome P450 isozyme belonging to the CYP2D subfamily and development of its antipeptide antibody, Nakamura A, Yamamoto Y, Tasaki T, Sugimoto C, Masuda M, Kazusaka A, Fujita S, Drug Metab Dispos23:1268-73Nov. 1995
  • Anti-peptide antibodies to the P4502D subfamily in rat, dog and man, (Nakamura A), Yamamoto Y, Tasaki T, Sugimoto C, Masuda M, Kazusaka A, Fujita S, Xenobiotica25:1103-1109:1103-1109Nov. 1995
  • Creating a Stable Short-term Housing Environment for Rabbits in a Cargo Van, Takafumi Tasaki, Masami Kojima, Yukihisa Suzuki, Yoshinori Tatematsu, HIroshi Sasaki, Journal of American Association for Laboratory Animal Science58:456-461Jul. 2019

Books etc

  • Japanese Encephalitis Virus Infection and Replication: Biological Roles of Nonstructural Protein NS4a and The 3'-Untranslated Region in Persistent Infection
    Japanese Encephalitis
    T.Takegami, T.Tasaki, M.Murakami, Y.Ishigaki, M.Taniguchi, T.Nojima, S.Nukuzuma
    SMGroup (USA)  Aug. 2015

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