SAKASAI Ryo

Researcher Profile & Settings

Research

Overview of research:
DNA damage responses induced by anti-cancer drugs

Special Field

• Risk sciences of radiation and chemicals

Degree

• Ph.D in Medicine

Research Activities

Published Papers

• 53BP1 regulates the self-renewal ability of neural stem/progenitor cells through modulating mitochondrial homeostasis, Yumi Sunatani, Ryo Sakasai, Tadashi Matsui, Kuniyoshi Iwabuchi, Biochemical and Biophysical Research Communications, 734:150776, Oct. 2024
• Camptothecin compromises transcription recovery and cell survival against cisplatin and ultraviolet irradiation regardless of transcription-coupled nucleotide excision repair, Ryo Sakasai, Mitsuo Wakasugi, Tadashi Matsui, Yumi Sunatani, Masafumi Saijo, Tsukasa Matsunaga, Kuniyoshi Iwabuchi, DNA Repair, 113:103318, Mar. 2022
  DOI:10.1016/j.dnarep
• Lens–specific conditional knockout of tropomyosin 1 gene in mice causes abnormal fiber differentiation and lens opacity., Teppei Shibata, Masahito Ikawa, Ryo Sakasai, Yasuhito Ishigaki, Etsuko Kiyokawa, Kuniyoshi Iwabuchi, Dhirendra P. Singh, Hiroshi Sasaki, Eri Kubo, Mechanisms of Ageing and Development, 196:111492, Apr. 2021
• USP42 enhances homologous recombination repair by promoting R-loop resolution with a DNA-RNA helicase DHX9, (Matsui Misaki), Sakasai Ryo, Abe Masako, Kimura Yusuke, Kajita Shoki, Torii Wakana, Katsuki Yoko, Ishiai Masamichi, Iwabuchi Kuniyoshi, Takata Minoru, Nishi Ryotaro, Oncogenesis, 9:60, Jun. 2020
  DOI:10.1038/s41389-020-00244-4
• Replication stress induces accumulation of FANCD2 at central region of large fragile genes, (Y.Okamoto),WM.Iwasaki, K.Kugou, KK.Takahashi, A.Oda, K.Sato, W.Kobayashi, H.Kawai, R.Sakasai, A.Takaori-Kondo, T.Yamamoto, MT.Kanemaki, M.Taoka, T.Isobe, K.Kurumizaka, H.Innan, K.Ohta, M.Ishiai, M.Takata, Nucleic acids research, 46:2932, Apr. 2018
  DOI:10.1093/nar/gky058
• Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase., Yumi.Sunatani, Radhika Pankaj Kamdar, Mukesh Kumar Sharma, Tadashi.Matsui, Ryo.Sakasai, Mitsumasa.Hashimoto, Yasuhito.Ishigaki, Yoshihisa.Matsumoto, Kuniyoshi.Iwabuchi, Experimental cell research, 362:450, Jan. 2018
  DOI:10.1016/j.yexcr.2017.12.009
• PARP inhibition disrupts repair of one-ended DNA double-strand breaks, Sakasai, Ryo;Iwabuchi, Kuniyoshi, CANCER SCIENCE, 109:577-577, Jan. 2018
• Aquarius is required for proper CtIP expression and homologous recombination repair., Sakasai Ryo;Isono Mayu;Wakasugi Mitsuo;Hashimoto Mitsumasa;Sunatani Yumi;Matsui Tadashi;Shibata Atsushi;Matsunaga Tsukasa;Iwabuchi Kuniyoshi, Scientific reports, 7:13808, 2017
  DOI:10.1038/s41598-017-13695-4
• Phosphorylation of EB2 by Aurora B and CDK1 ensures mitotic progression and genome stability., (M.Iimori), S.Watanabe, S.Kiyonari,　K.Matsuoka, R.Sakasai, H.Saeki, E.Oki, H.Kitao, Y.Maehara, Nature Communications, 7:11117-11117, 2016
• The distinctive cellular responses to DNA strand breaks caused by a DNA topoisomerase I poison in conjunction with DNA replication and RNA transcription., R.Sakasai, K.Iwabuchi, Genes & genetic systems, 90:187-194, 2015
  DOI:10.1266/ggs.15-00023
• Arf/p53 module, which induces apoptosis, downregulates histone H2AX to allow normal cells to survive in the presence of anti-cancer drugs, (Atsumi Y), Inase A, Osawa T, Sugihara E, Sakasai R, Fujimori H, Teraoka H, Saya H, Kanno M, Nakagama H, Masutani M, Yoshioka KI, The Journal of biological chemistry., 288:13269-13277, 2013
• PARP and CSB modulate the processing of transcription-mediated DNA strand breaks, Sakai A, Sakasai R, Kakeji Y, Kitao H, Maehara Y, Genes & genetic systems., 87:265-272, 2012
• CtIP- and ATR-dependent FANCJ phosphorylation in response to DNA strand breaks mediated by DNA replication, Sakasai R, Sakai A, Iimori M, Kiyonari S, Matsuoka K, Kakeji Y, *Kitao H, Maehara Y, Kakeji Y, Kitao H, Maehara Y, Genes to cells : devoted to molecular & cellular mechanisms, 17:962-970, 2012
• ATR-Chk1 signaling pathway and homologous recombinational repair protect cells from 5-fluorouracil cytotoxicity, (Fujinaka Y), Matsuoka K, Iimori M, Tuul M, Sakasai R, Yoshinaga K, Saeki H, Morita M, Kakeji Y, Gillespie DA, Yamamoto K, Takata M, Kitao H, Maehara Y, DNA repair., 11:247-158, 2012
• Transcription-dependent activation of ataxia telangiectasia-mutated prevents DNA-dependent protein kinase-mediated cell death in response to topoisomerase I poison, Sakasai R, Teraoka H, Tibbetts RS, The Journal of biological chemistry., 285:15201-15208, 2010
• Proteasome inhibition suppresses DNA-dependent protein kinase activation caused by camptothecin, Sakasai R, Teraoka H, Tibbetts RS, DNA repair., 9:76-82, 2010
• DPPA4 modulates chromatin structure via association with core histone H3 and DNA in mouse embryonic stem cells, (Masaki H), Nishida T, Sakasai R, Teraoka H, Genes to cells : devoted to molecular & cellular mechanisms, 15:327-337, 2010
• Potentiation of ALS-associated TDP-43 aggregation by the proteasome-targeting factor, Ubiquilin 1, (Kim SH), Shi Y, Hanson KA, Williams LM, Sakasai R, Bowler MJ, Tibbetts RS, The Journal of biological chemistry., 284:8083-8092, 2009
• RNF8-dependent and independent activation of 53BP1 in response to DNA damage, Sakasai R, Tibbetts RS, The Journal of biological chemistry., 283:13549-13555, 2008
• Differential involvement of phosphatidylinositol 3-kinase- related kinases in replication protein A2 hyperphosphorylation in response to replication-mediated DNA double-strand breaks, Sakasai R, Shinohe K, Ichijima Y, Okita N, Shibata A, Asahina K, Teraoka H, Genes to cells : devoted to molecular & cellular mechanisms, 11:237-246, 2006
• Simultaneous Injection of Contrast and Saline Using Spiral Flow-Generating Tube for Hepatic Dynamic Computed Tomography: Effect on Enhancement of Liver Parenchyma and Metastases to the Liver, Hiroji Nagata,Hisako Iori,Shiori Yoshida,Hiroki Kawashima, Yuka Nishino,Ryo Sakasai, Hiroshi Yamamura, Tetsuya Minami, Journal of computer assisted tomography., 48:875-881, Nov. 2024