Japanese

NISHIZONO Hirofumi

■Faculty	Research Support Center
■Position	Associate Professor

Researcher Profile & Settings

Research

Overview of research:

Our research focus is on the relationship between the oviduct, which is the first microenvironment we experience, and mammalian embryos, with a particular interest in signal transduction via Cys-loop receptors (Nishizono et al, Reproduction 2020). We are also working on the development of genome editing tools such as CRISPR-Cas9 (Nishizono et al, Molecular Brain 2021).

Special Field

Obstetrics and gynecology, Developmental biology

Degree

Research Activities

Published Papers

Tle6 deficiency in male mice led to abnormal sperm morphology and reduced sperm motility., Kazama Kousuke, Miyagoshi Yuki, Nishizono Hirofumi, Frontiers in cell and developmental biology, 12:1481659, Oct. 2024
DOI:10.3389/fcell.2024.1481659
Large-scale animal model study uncovers altered brain pH and lactate levels as a transdiagnostic endophenotype of neuropsychiatric disorders involving cognitive impairment., (Hideo Hagihara), Hirotaka Shoji, Satoko Hattori, Giovanni Sala, Yoshihiro Takamiya, Mika Tanaka, Masafumi Ihara, Mihiro Shibutani, Izuho Hatada, Kei Hori, Mikio Hoshino, Akito Nakao, Yasuo Mori, Shigeo Okabe, Masayuki Matsushita, Anja Urbach, Yuta Katayama, Akinobu Matsumoto, Keiichi I Nakayama, Shota Katori, Takuya Sato, Takuji Iwasato, Haruko Nakamura, Yoshio Goshima, Matthieu Raveau, Tetsuya Tatsukawa, Kazuhiro Yamakawa, Noriko Takahashi, Haruo Kasai, Johji Inazawa, Ikuo Nobuhisa, Tetsushi Kagawa, Tetsuya Taga, Mohamed Darwish, Hirofumi Nishizono, Keizo Takao, Kiran Sapkota, Kazutoshi Nakazawa, Tsuyoshi Takagi, Haruki Fujisawa, Yoshihisa Sugimura, Kyosuke Yamanishi, Lakshmi Rajagopal, Nanette Deneen Hannah, Herbert Y Meltzer, Tohru Yamamoto, Shuji Wakatsuki, Toshiyuki Araki, Katsuhiko Tabuchi, Tadahiro Numakawa, Hiroshi Kunugi, Freesia L Huang, Atsuko Hayata-Takano, Hitoshi Hashimoto, Kota Tamada, Toru Takumi, Takaoki Kasahara, Tadafumi Kato, Isabella A Graef, Gerald R Crabtree, Nozomi Asaoka, Hikari Hatakama, Shuji Kaneko, Takao Kohno, Mitsuharu Hattori, Yoshio Hoshiba, Ryuhei Miyake, Kisho Obi-Nagata, Akiko Hayashi-Takagi, Léa J Becker, Ipek Yalcin, Yoko Hagino, Hiroko Kotajima-Murakami, Yuki Moriya, Kazutaka Ikeda, Hyopil Kim, Bong-Kiun Kaang, Hikari Otabi, Yuta Yoshida, Atsushi Toyoda, Noboru H Komiyama, Seth GN Grant, Michiru Ida-Eto, Masaaki Narita, Ken-ichi Matsumoto, Emiko Okuda-Ashitaka, Iori Ohmori, Tadayuki Shimada, Kanato Yamagata, Hiroshi Ageta, Kunihiro Tsuchida, Kaoru Inokuchi, Takayuki Sassa, Akio Kihara, Motoaki Fukasawa, Nobuteru Usuda, Tayo Katano, Teruyuki Tanaka, Yoshihiro Yoshihara, Michihiro Igarashi, Takashi Hayashi, Kaori Ishikawa, Satoshi Yamamoto, Naoya Nishimura, Kazuto Nakada, Shinji Hirotsune, Kiyoshi Egawa, Kazuma Higashisaka, Yasuo Tsutsumi, Shoko Nishihara, Noriyuki Sugo, Takeshi Yagi, Naoto Ueno, Tomomi Yamamoto, Yoshihiro Kubo, Rie Ohashi, Nobuyuki Shiina, Kimiko Shimizu, Sayaka Higo-Yamamoto, Katsutaka Oishi, Hisashi Mori, Tamio Furuse, Masaru Tamura, Hisashi Shirakawa, Daiki X Sato, Yukiko U Inoue, Takayoshi Inoue, Yuriko Komine, Tetsuo Yamamori, Kenji Sakimura, Tsuyoshi Miyakawa, eLife, 12:RP89376, Mar. 2024
DOI:10.7554/eLife.89376
Comprehensive behavioral analyses of mice with a glycine receptor alpha 4 deficiency., (Mohamed Darwish), Satoko Hattori, Hirofumi Nishizono, Tsuyoshi Miyakawa, Nozomu Yachie, Keizo Takao, Molecular Brain, 16:44, May 2023
Survey of the Review Process for Approved Animal Protocols and Feedback to Education and Training., Shintaro Matsuba, Ryoya Yamamoto, Yasuhito Ishigaki, Hirofumi Nishizono, JOURNAL OF KANAZAWA MEDICAL UNIVERSITY, 48:81-84, Dec. 2023
In vitro survival kinetics of microfluidic-sorted bovine spermatozoa, Ogata K, Nagata MPB, Nishizono H, Yamanouchi T, Matsuda H, Ogata Y, Takeda K, Hashiyada Y, Yamashita K., Andrology, 9:977-988, May 2021
Rapid generation of conditional knockout mice using the CRISPR-Cas9 system and electroporation for neuroscience research, Nishizono H, Hayano Y, Nakahata Y, Ishigaki Y, Yasuda R., Molecular Brain, 14:148, Jul. 2021
Decrease of fertilizing ability of mouse spermatozoa after freezing and thawing is related to cellular injury., Nishizono Hirofumi;Shioda Masaki;Takeo Toru;Irie Tetsumi;Nakagata Naomi, Biology of reproduction, 71, 2004
Hippocampal function is not required for the precision of remote place memory., Kitamura Takashi;Okubo-Suzuki Reiko;Takashima Noriko;Murayama Akiko;Hino Toshiaki;Nishizono Hirofumi;Kida Satoshi;Inokuchi Kaoru, Molecular brain, 5, 2012
DOI:10.1186/1756-6606-5-5
Role of transient receptor potential vanilloid 4 activation in indomethacin-induced intestinal damage., Yamawaki Hidemoto;Mihara Hiroshi;Suzuki Nobuhiro;Nishizono Hirofumi;Uchida Kunitoshi;Watanabe Shiro;Tominaga Makoto;Sugiyama Toshiro, American journal of physiology. Gastrointestinal and liver physiology, 307, 2014
DOI:10.1152/ajpgi.00105.2013
Artificial association of pre-stored information to generate a qualitatively new memory., Ohkawa Noriaki;Saitoh Yoshito;Suzuki Akinobu;Tsujimura Shuhei;Murayama Emi;Kosugi Sakurako;Nishizono Hirofumi;Matsuo Mina;Takahashi Yukari;Nagase Masashi;Sugimura Yae K;Watabe Ayako M;Kato Fusao;Inokuchi Kaoru, Cell reports, 11, 2015
DOI:10.1016/j.celrep.2015.03.017
Protease-Activated Receptor-2 Up-Regulates Transient Receptor Potential Vanilloid 4 Function in Mouse Esophageal Keratinocyte., Suzuki Nobuhiro;Mihara Hiroshi;Nishizono Hirofumi;Tominaga Makoto;Sugiyama Toshiro, Digestive diseases and sciences, 60, 2015
DOI:10.1007/s10620-015-3822-6
PDGFRα plays a crucial role in connective tissue remodeling., Horikawa Shinjiro;Ishii Yoko;Hamashima Takeru;Yamamoto Seiji;Mori Hisashi;Fujimori Toshihiko;Shen Jie;Inoue Ran;Nishizono Hirofumi;Itoh Hiroshi;Majima Masataka;Abraham David;Miyawaki Toshio;Sasahara Masakiyo, Scientific reports, 5, 2015
DOI:10.1038/srep17948
Glioma-Derived Platelet-Derived Growth Factor-BB Recruits Oligodendrocyte Progenitor Cells via Platelet-Derived Growth Factor Receptor-α and Remodels Cancer Stroma., Zheng Yang;Yamamoto Seiji;Ishii Yoko;Sang Yang;Hamashima Takeru;Van De Nguyen;Nishizono Hirofumi;Inoue Ran;Mori Hisashi;Sasahara Masakiyo, The American journal of pathology, 186, 2016
DOI:10.1016/j.ajpath.2015.12.020
Cellular tagging as a neural network mechanism for behavioural tagging., Nomoto Masanori;Ohkawa Noriaki;Nishizono Hirofumi;Yokose Jun;Suzuki Akinobu;Matsuo Mina;Tsujimura Shuhei;Takahashi Yukari;Nagase Masashi;Watabe Ayako M;Kato Fusao;Inokuchi Kaoru, Nature communications, 7, 2016
DOI:10.1038/ncomms12319
Overlapping memory trace indispensable for linking, but not recalling, individual memories., Yokose Jun;Okubo-Suzuki Reiko;Nomoto Masanori;Ohkawa Noriaki;Nishizono Hirofumi;Suzuki Akinobu;Matsuo Mina;Tsujimura Shuhei;Takahashi Yukari;Nagase Masashi;Watabe Ayako M;Sasahara Masakiyo;Kato Fusao;Inokuchi Kaoru, Science (New York, N.Y.), 355, 2017
DOI:10.1126/science.aal2690
Cleavage Speed and Blastomere Number in DBA/2J Compared with C57BL/6J Mouse Embryos., Nishizono Hirofumi;Uno Kyosuke;Abe Hiroyuki, Journal of the American Association for Laboratory Animal Science : JAALAS, 56, 2017
Live births from artificial insemination of microfluidic-sorted bovine spermatozoa characterized by trajectories correlated with fertility., Nagata Maria Portia B;Endo Kenji;Ogata Kazuko;Yamanaka Kenichi;Egashira Junki;Katafuchi Naoto;Yamanouchi Tadayuki;Matsuda Hideo;Goto Yuki;Sakatani Miki;Hojo Takuo;Nishizono Hirofumi;Yotsushima Kenji;Takenouchi Naoki;Hashiyada Yutaka;Yamashita Kenichi, Proceedings of the National Academy of Sciences of the United States of America, 115, 2018
DOI:10.1073/pnas.1717974115
Autophagy Enhances Memory Erasure through Synaptic Destabilization., Shehata Mohammad;Abdou Kareem;Choko Kiriko;Matsuo Mina;Nishizono Hirofumi;Inokuchi Kaoru, The Journal of neuroscience : the official journal of the Society for Neuroscience, 38, 2018
DOI:10.1523/JNEUROSCI.3505-17.2018
CD11c+ M1-like macrophages (MΦs) but not CD206+ M2-like MΦ are involved in folliculogenesis in mice ovary., Ono Yosuke;Nagai Miwako;Yoshino Osamu;Koga Kaori;Nawaz Allah;Hatta Hideki;Nishizono Hirofumi;Izumi Gentaro;Nakashima Akitoshi;Imura Johji;Tobe Kazuyuki;Fujii Tomoyuki;Osuga Yutaka;Saito Shigeru, Scientific reports, 8, 2018
DOI:10.1038/s41598-018-25837-3
Synapse-specific representation of the identity of overlapping memory engrams., Abdou Kareem;Shehata Mohammad;Choko Kiriko;Nishizono Hirofumi;Matsuo Mina;Muramatsu Shin-Ichi;Inokuchi Kaoru, Science (New York, N.Y.), 360, 2018
DOI:10.1126/science.aat3810
Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification., Sadahiro Taketaro;Isomi Mari;Muraoka Naoto;Kojima Hidenori;Haginiwa Sho;Kurotsu Shota;Tamura Fumiya;Tani Hidenori;Tohyama Shugo;Fujita Jun;Miyoshi Hiroyuki;Kawamura Yoshifumi;Goshima Naoki;Iwasaki Yuka W;Murano Kensaku;Saito Kuniaki;Oda Mayumi;Andersen Peter;Kwon Chulan;Uosaki Hideki;Nishizono Hirofumi;Fukuda Keiichi;Ieda Masaki, Cell stem cell, 23, 2018
DOI:10.1016/j.stem.2018.07.001
Artificial association of memory events by optogenetic stimulation of hippocampal CA3 cell ensembles., Oishi Naoya;Nomoto Masanori;Ohkawa Noriaki;Saitoh Yoshito;Sano Yoshitake;Tsujimura Shuhei;Nishizono Hirofumi;Matsuo Mina;Muramatsu Shin-Ichi;Inokuchi Kaoru, Molecular brain, 12, 2019
DOI:10.1186/s13041-018-0424-1
Rapid and high-efficient generation of mutant mice using freeze-thawed embryos of the C57BL/6J strain., Darwish Mohamed;Nishizono Hirofumi;Uosaki Hideki;Sawada Hitomi;Sadahiro Taketaro;Ieda Masaki;Takao Keizo, Journal of neuroscience methods, 317, 2019
DOI:10.1016/j.jneumeth.2019.01.010
Orchestrated ensemble activities constitute a hippocampal memory engram., Ghandour Khaled;Ohkawa Noriaki;Fung Chi Chung Alan;Asai Hirotaka;Saitoh Yoshito;Takekawa Takashi;Okubo-Suzuki Reiko;Soya Shingo;Nishizono Hirofumi;Matsuo Mina;Osanai Makoto;Sato Masaaki;Ohkura Masamichi;Nakai Junichi;Hayashi Yasunori;Sakurai Takeshi;Kitamura Takashi;Fukai Tomoki;Inokuchi Kaoru, Nature communications, 10, 2019
DOI:10.1038/s41467-019-10683-2
Glycine receptor α4 subunit facilitates the early embryonic development in mice., Nishizono Hirofumi;Darwish Mohamed;Endo Takaho A;Uno Kyosuke;Abe Hiroyuki;Yasuda Ryohei, Reproduction (Cambridge, England), 159, 2020
DOI:10.1530/REP-19-0312
Pcdhβ deficiency affects hippocampal CA1 ensemble activity and contextual fear discrimination., Asai Hirotaka;Ohkawa Noriaki;Saitoh Yoshito;Ghandour Khaled;Murayama Emi;Nishizono Hirofumi;Matsuo Mina;Hirayama Teruyoshi;Kaneko Ryosuke;Muramatsu Shin-Ichi;Yagi Takeshi;Inokuchi Kaoru, Molecular brain, 13, 2020
DOI:10.1186/s13041-020-0547-z
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice., Nishizono Hirofumi;Darwish Mohamed;Uosaki Hideki;Masuyama Nanami;Seki Motoaki;Abe Hiroyuki;Yachie Nozomu;Yasuda Ryohei, Journal of visualized experiments : JoVE, 2020
DOI:10.3791/60808
Methodologies and Challenges for CRISPR/Cas9 Mediated Genome Editing of the Mammalian Brain., Nishizono Hirofumi;Yasuda Ryohei;Laviv Tal, Frontiers in genome editing, 2, 2020
DOI:10.3389/fgeed.2020.602970

Note: Performance information for the past 1-2 years may be revised at a later date due to inspections conducted by the committee from July to September every year.