IWABUCHI Kuniyoshi

FacultyDepartment of Biochemistry Ⅰ

Researcher Profile & Settings



    Overview of research:
    Roles of tumor suppressor p53-binding protein 1 (53BP1) in the cell cycle checkpoint, and repair of DNA double-strand breaks.


  • Kanazawa University Graduate School
  • Kanazawa University Faculty of Medicine Department of Medicine


  • Medical doctor

Research Activities

Published Papers

  • Camptothecin compromises transcription recovery and cell survival against cisplatin and ultraviolet irradiation regardless of transcription-coupled nucleotide excision repair, Ryo Sakasai, Mitsuo Wakasugi, Tadashi Matsui, Yumi Sunatani, Masafumi Saijo, Tsukasa Matsunaga, Kuniyoshi Iwabuchi, DNA Repair113:103318Mar. 2022
  • Identification of CD56 dim subpopulation marked with high expression of GZMB/PRF1/PI-9 in CD56 + interferon-α-induced dendritic cells, Asuka Watanabe, Misa Togi, Terutsugu Koya, Makoto Taniguchi , Takuya Sakamoto, Kuniyoshi Iwabuchi, Tomohisa Kato, Shigetaka Shimodaira, Genes to cells26:313-327May 2021
  • Lens–specific conditional knockout of tropomyosin 1 gene in mice causes abnormal fiber differentiation and lens opacity., Teppei Shibata, Masahito Ikawa, Ryo Sakasai, Yasuhito Ishigaki, Etsuko Kiyokawa, Kuniyoshi Iwabuchi, Dhirendra P. Singh, Hiroshi Sasaki, Eri Kubo, Mechanisms of Ageing and Development196:111492Apr. 2021
  • DNA damage in human glomerular endothelial cells induces nodular glomerulosclerosis via an ATR and ANXA2 pathway, Fujii A, Sunatani Y, Furuichi K, Fujimoto K, Adachi H, Iwabuchi K, Yokoyama H, Scientific Reports10:22206Dec. 2020
  • USP42 enhances homologous recombination repair by promoting R-loop resolution with a DNA-RNA helicase DHX9, (Matsui Misaki), Sakasai Ryo, Abe Masako, Kimura Yusuke, Kajita Shoki, Torii Wakana, Katsuki Yoko, Ishiai Masamichi, Iwabuchi Kuniyoshi, Takata Minoru, Nishi Ryotaro, Oncogenesis9:60Jun. 2020
  • Preliminary quantitative evaluation of radiation-induced DNA damage in peripheral blood lymphocytes after cardiac dual-isotope imaging, Koichi Okuda, Naoto Watanabe, Mitsumasa Hashimoto, Mariko Doai, Yasuyuki Kawai, Tomoko Takahashi, Tomohiro Arikawa, Kazumasa Ooiso, Yumi Sunatani, Kuniyoshi Iwabuchi, Koji Kajinami, Munetaka Matoba, Applied Radiation and Isotopes154:108890Dec. 2019
  • Caspase-mediated cleavage of X-ray repair cross-complementing group 4 promotes apoptosis by enhancing nuclear translocation of caspase-activated DNase., Yumi.Sunatani, Radhika Pankaj Kamdar, Mukesh Kumar Sharma, Tadashi.Matsui, Ryo.Sakasai, Mitsumasa.Hashimoto, Yasuhito.Ishigaki, Yoshihisa.Matsumoto, Kuniyoshi.Iwabuchi, Experimental cell research362:450Jan. 2018
  • PARP inhibition disrupts repair of one-ended DNA double-strand breaks, Sakasai, Ryo;Iwabuchi, Kuniyoshi, CANCER SCIENCE109:577-577Jan. 2018
  • Aquarius is required for proper CtIP expression and homologous recombination repair, Ryo.Sakasai, Mayu.Isono, Mitsuo.Wakasugi, Mitsumasa.Hashimoto, Yumi.Sunatani, Tadashi.Matsui, Atsushi.Shibata., Tsukasa.Matsunaga, Kuniyoshi.Iwabuchi, SCIENTIFIC REPORTS7:13808Oct. 2017
  • Csapase-3/7-mediated Cleavage of β2-spectrinis Required for Acctaminophen-induced Liver Damage, (HJ.Baek), YM.Lee, TH.Kim, JY.Kim, FJ.Park, K.Iwabuchi, L.Mishra, SS.Kim12:1722016
  • DNA double-strand breaks induced intractable glomerular fibrosis in renal allografts., (Y.Matsui), Y.Sunatani, N.Hayashi, K.Okino, Y.Okushi, K.Mukai, H.Adachi, H.Yamaya, K.Iwabuchi, H.Yokoyama, Clinical and experimental nephrology20:479-4882015
  • The distinctive cellular responses to DNA strand breaks caused by a DNA topoisomerase I poison in conjunction with DNA replication and RNA transcription., R.Sakasai, K.Iwabuchi, Genes & genetic systems90:187-1942015
  • Protein kinase CK2 is required for the recruitment of 53BP1 to sites of DNA double-strand break induced by radiomimetic drugs., (B.Guerra), K.Iwabuchi, OG.Issinger, Cancer letters.345:115-123Apr. 2014
  • DHX36 enhances RIG-I signaling by facilitating PKR-mediated antiviral stress granule formation., (JS.Yoo), K.Takahasi, CS.Ng, R.Ouda, K.Onomoto, M.Yoneyama, JC.Lai, S.Lattmann, Y.nagamine, T.Matsui, K.Iwabuchi, H.Kato, T.Fujita, PLoS pathogens.10:e1004012;1-18Mar. 2014
  • RNA-binding protein RBM8A (Y14) and MAGOH localize to centrosome in human A549 cells., Y.Ishigaki, Y.Nakamura, T.Tatsuno, M.Hashimoto, K.Iwabuchi, N.Tomosugi, Histochemistry and cell biology.141:101-109Jan. 2014
  • Sensitive immunodetection of radiotoxicity after iodine-131therapy for thyroid cancer using γ-H2AX foci of DNA damage in lymphocytes., M.Doai, N.Watanabe, T.Takahashi, M.Taniguchi, H.Tonami, K.Iwabuchi, D.Kayano, M.Fukuoka, S.Kinuya, Annals of nuclear medicine.27:233-238Apr. 2013
  • Depletion of RNA-binding protein RBM8A (Y14) causes cell cycle deficiency and apoptosis in human cells., Y.Ishigaki, Y.Nakamura, T.Tatsuno, M.Hashimoto, T.Shimasaki, K.Iwabuchi, N.Tomosugi, Experimental biology and medicine (Maywood, N.J.)238:889-8972013
  • DNA ligase IV and artemis act cooperatively to suppress homologous recombination in human cells: Implications for DNA double-strand break repair., (A.Kurosawa), S.Saito, S.So, M.Hashimoto, K.Iwabuchi, H.Watabe, N.Adachi, PloS one8:e72253;1-112013
  • Knockdown of stromal interaction molecule 1 (STIM1) suppresses store-operated calcium entry, cell proliferation and tumorigenicity in human epidermoid carcinoma A431 cells, J.Yoshida, K.Iwabuchi, T.Matsui, T.Ishibashi, T.Masuoka, M.Nishio, Biochemical pharmacology.84:1592-1603Sep. 2012
  • RAD18 promotes DNA double-strand break repair during G1 phase through chromatin retention of 53BP1, (K.Watanabe), K.Iwabuchi, J.Sun, Y.Tsuji, T.Tani, K.Tokunaga, T.Date, M.Hashimoto, M.Yamaizumi, S.Tateishi, Nucleic acids research37:2176-2193Jan. 2009
  • PKU-β/TLK1 regulates myosin Ⅱ activities, and is required for accurate equaled chromosome segregation, M.Hashimoto, T.Matsui, K.Iwabuchi, T.Date, Mutation research657:63-67Sep. 2008
  • Characterization of a cancer cell line that expresses a splicing variant form of 53BP1:Separation of checkpoint and repair functions in 53BP1, K.Iwabuchi, T.Matsui, M.Hashimoto, Y.Matsumoto, T.Kurihara, T.Date, Biochemical and biophysical research communications376:509-513Sep. 2008
  • Cell sorting analysis of cell cycle-dependent X-ray sensitivity in end joining-deficient human cells, K.Iwabuchi, M.Hashimoto, T.Matsui, A.Kurosawa, N.Adachi, T.Date, Biochemical and biophysical research communications372:662-667May 2008
  • Current topics in DNA double-strand break repair, (J.Kobayashi), K.Iwabuchi, K.Miyagawa, E.Sonoda, K.Suzuki, M.Takata, H.Tauchi, Journal of radiation research49:93-1032008
  • Perturbed gap-filling synthesis in nucleotide excision repair causes histone H2AX phosphorylation in human quiescent cells, (M.Matsumoto), K.Yaginuma, A.Igarashi, M.Imura, M.Hasegawa, K.Iwabuchi, T.Date, T.Mori, K.Ishizaki, K.Yamashita, M.Inobe, T.Matsunaga, Journal of cell science6:1104-1112Jan. 2007
  • 53BP1 contributes to survival of cells irradiated with X-ray during G1 without Ku70 or Artemis., K.Iwabuchi, M.Hashimoto, T.Matsui, T.Kurihara, H.Shimizu, N.Adachi, M.Ishiai, K-I.Yamamoto, H.Tauchi, M.Takata, H.Koyama, T.Date, Genes to cells : devoted to molecular & cellular mechanisms11: 935-948Aug. 2006
  • Induction of centrosome amplification in p53 siRNA-treated human fibroblast cells by radiation exposure, K.Kawamura, N.Morita, C.Domiki, K.Fujikawa-Yamamoto, M.Hashimoto, K.Iwabuchi, K.Suzuki, Cancer science.97:252-2582006
  • Hepatitis C virus core protein interacts with p53-bindinng protein,53BP2/Bbp/ASPP2,and inhibits p53-mediated apoptosis, Y.Cao, T.Hamada, T.Matsui, T.Date, K.Iwabuchi, Biochem. Biophys. Res. Commun.315:788-7952004
  • Centrosome hyperamplification and chromosomal damage after exposuer to radiation, *K.Kawamura, *K.Fujikawa-Yamamoto, *M.Ozaki, K.Iwabuchi, *H.Nakashima, *C.Domiki, *N.Morita, *M.Inoue, *K.Tokunaga, *N.Shiba, *R.Ikeda, *K.Suzuki, Oncology.67:460-4702004
  • ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation, (T.Stiff), K.Iwabuchi, et al., Cancer research.64:2390-23962004
  • Potential role for 53BP1 in DNA end-joining repair through direct interaction with DNA, K. Iwabuchi, T. Hamada, *T. Kurihara, T. Date, et al., The Journal of biological chemistry.278: 36487-364952003
  • Purification, crystallization and preliminary x-ray analysis of a complex of the BRCT domains of human 53BP1 bound to the p53 tumour suppressor, (Derbyshire, D. J.), T. Date, K. Iwabuchi, et al.58: 1826-18292002
  • Crystal structure of the BRCT domains of human 53BP1 bound to the p53 tumor suppressor, (Derbyshire, D. J.), T.Date, K. Iwabuchi, et al., The EMBO journal.21: 3863-38722002
  • Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathway, (I. Rappold), K. Iwabuchi, T. Date, et al., The Journal of cell biology.153: 613-6202001
  • Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2(共著), IWABUCHI Kuniyoshi, J. Biol. Chem.273:26061-260681998
  • Nodal EBV-positive Hudgkin's disease following extranodal EBV megative non-Hodgkin's lymphoma of B-cell lineage(共著), IWABUCHI Kuniyoshi, European Journal of Haematology1996
  • In situ localization of Epstein-Barr Virus in Reed-Sternberg Cells of Hodgkin's Disease(共著), IWABUCHI Kuniyoshi, Journal of Kanazawa medical university1996
  • Two cellular proteins that bind to wild-type but not mutant p53. (共著), IWABUCHI Kuniyoshi, Proc. Nati. Acad. Sci. USA.91:6098-61021994
  • Use of the two-hybrid system to identify the domain of p53 involved in oligomerization(共著), IWABUCHI Kuniyoshi, oncogene8:1693-16961993
  • Plasma glycosyl transferase activity after ABO-incompatible bone marrow transplantation and development of an inhibitor for glycosyltransferase activity(共著), IWABUCHI Kuniyoshi, Experimental hematology1989
  • Long-term observation of immunologic reconstitution after allogenic bone marrow transplantation : Differences in recovery among functional T cell subsets(共著), IWABUCHI Kuniyoshi, Acta Haematologica Japonica1989